Witryna23 lis 2024 · On day 12 of development, cerebral organoid cultures were transferred from orbital shakers (Fig. 5A) to automated microfluidic wells (Fig. 5B). The orbital shaker maintains a quasi-steady velocity ... Witryna18 mar 2024 · Use a horizontal shaker to agitate the multi-well plate(s) for 5 min at 600 rpm. CRITICAL: ... If the organoid cultures show a change in growth speed during the expansion phase, we recommend to replicate the growth curve experiment to assess the best organoid seeding density. To avoid a heterogeneous dispensing of the …

Modular automated microfluidic cell culture platform reduces …

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Mouse-Derived Gastric Organoid and Immune Cell Co-culture …

Witryna7 mar 2024 · At day 7, the monolayer cultures of IM cells were exposed to EDTA or TrypLE Select and the resulting cell suspension was subjected to low speed (60 rpm) swirling on an orbital shaker in the presence of differentiation media (basal media that contains FGF9+heparin±CHIR99021) with 0.1% polyvinyl alcohol (PVA) and methyl … WitrynaA culture medium for hepatoma organoid culture, comprising an MST1/2 kinase inhibitor, at least one cell culture additive selected from N2 and B27, a hepatocyte growth factor, an ITS cell culture additive, Y27632, dexamethasone, neuromodulation protein 1, insulin, an epidermal cell growth factor, GlutaMAX, and a non-essential … WitrynaPlace plate of organoids on an orbital shaker in a 37°C incubator. For the INFORS HT Celltron orbital shaker, set the shaker speed (rpm) according to Table 1. For other … french broad chocolate north carolina