Shrna fancl
SpletshRNA gene knockout and verification kit; sgRNA gene knockout kit; Stable cell line; Promoter Reporters. Promoter selection plates; Ubiquitous promoter reporters; Tissue … SpletNational Center for Biotechnology Information
Shrna fancl
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SpletUse shRNA set against Chicken FANCL (NM_001033941) for functional genomics research and drug discovery study. 2024-nCoV Research Solution Guide Monkeypox Virus Real … Splet19. jun. 2013 · FANCL ubiquitinates β-catenin with atypical ubiquitin chains (K11 linked), a modification that functionally enhances β-catenin activity. Suppression of FANCL …
SpletPredesigned shRNA. 1. Search Genes/Clones. 2. Select Clones. 3. Specifications. 4. Review & Confirm. Help. [email protected]. 080-66219600. Manual Search Upload … SpletA short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi). Expression of shRNA in cells is typically accomplished by delivery of plasmids or through viral or bacterial vectors. shRNA is an advantageous mediator of …
Splet27. jun. 2024 · shRNA实验步骤 NANAXI_67da 关注 IP属地: 广东 0.591 2024.06.27 01:05:35 字数 1,445 阅读 13,839 试剂 image.png 实验步骤 1.合成Nestin干扰序列 2.干扰序列退火 将正反向干扰序列以10μM浓度溶于ddH2O，按以下体系将正反向干扰序列退火形成双链粘末端DNA： 10 μL Forward oligo 10 μL Reverse oligo 5 μL 10x NEB buffer 2 25 μL ddH2O 置 … Splet产品技术背景pri系列载体是基于iii类rna聚合酶启动子：人类h1启动子的专用于哺乳动物细胞rna干扰的载体。h1启动子在哺乳动物细胞内合成类似sirna分子的小分子rna。由于h1启动子有精确的转录起始位点和终止信号，h1启动子转录产物精确生成人工设计的shrna，shrna经过risc剪切后形成有2个u突出末端的 ...
Splet2. Ligate the shRNA Template into Linearized shRNA Lentivector a. Set up 10 µl ligation reactions for each phosphorylated shRNA template as follows: 1.0 µl Linearized shRNA Expression Vector (10 ng/µl) (see Required Materials) 0.5 µl Phosphorylated ds shRNA template (step 1; 0.2 µM) * 1.0 µl 10X T4 DNA Ligase Buffer 6.5 µl Deionized water
SpletFANCL siRNA (m), shRNA und Lentivirale Partikel Gen-Silencer sind für das Herunterregulieren der Genexpression des murinen FANCL konzipiert Wählen Sie eine … tickets to la lakers gameSpletFANCL shRNA constructs labeled A, B, and C were used for subsequent experiments. (B) Importantly, suppression of FANCL expression reproduces the classic FA phenotype of … tickets to kennedy space center flSpletshRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene … tickets to lahoreSpletPredesigned shRNA. 1. Search Genes/Clones. 2. Select Clones. 3. Specifications. 4. Review & Confirm. Help. [email protected]. 1-800-234-5362. Manual Search Upload … the lodge traverse citySplet06. mar. 2024 · shRNA： 小发卡或短发卡RNA (a small hairpin RNA or short hairpin RNA，shRNA)是一段具有紧密发卡环 (tight hairpin turn)的RNA序列，常被用于RNA干扰沉默靶基因的表达。 利用载体把shRNA导入细胞，载体中的U6启动子确保shRNA总是表达；shRNA的发卡结构可被细胞机制切割成siRNA，然后siRNA结合到RNA诱导沉默复合物 … the lodge tuddenhamSplet25. jul. 2009 · Methods of mediating the RNAi effect involve small interfering RNA (siRNA), short hairpin RNA (shRNA) and bi-functional shRNA. The simplicity of siRNA manufacturing and transient nature of the effect per dose are optimally suited for certain medical disorders (i.e. viral injections). However, using the endogenous processing machinery, optimized ... the lodge tucson arizonaSpletComprehensive - Trusted TRC (The RNAi Consortium) content with shRNA taregting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma. Universal - Simplified whole genome RNAi screening in standard or difficult to expand cell lines. Versatile - Compatible with FACS and most ... tickets to last supper in milan